Scientist I/II – RNA Biochemistry

Posted on January 7,

Skyhawk Therapeutics is seeking a highly motivated RNA Biochemistry Scientist I/II to join our talented, growing Molecular Biology team in Waltham, MA. The successful candidate will develop and implement new RNA biochemical tools to drive mechanism of action studies of Skyhawk’s RNA-targeting small molecules and support advancing drug discovery programs. The candidate will have strong experimental skills and deep knowledge in molecular mechanisms of RNA biology. As a member of an interdisciplinary platform team, the candidate will contribute to the innovative development of Skyhawk platform technologies at the interface of chemistry, RNA biochemistry and structural biology. 

Skyhawk is committed to discovering, developing and commercializing small molecule therapeutics that correct RNA splicing. We use our novel SkySTARTM platform (Skyhawk Small molecule Therapeutics for Alternative splicing of RNA) to develop drug candidates directed to targets for some of the world’s most intractable diseases including cancer, neurological conditions, and other “undruggable” targets against a wide range of conditions.

Role and Responsibilities (include, but are not limited to):

  • Design and validate reporter constructs for in vitro and cell-based assays to monitor RNA processing.
  • Design and perform in vitro biochemical assays for high-throughput hit discovery and medium-throughput

hit-to-lead optimization.

  • Develop and build a suite of in vitro and cell-based biochemical tools to elucidate mechanism of action of splicing modulators.
  • Utilize in vitro and cellular proteomics approaches to investigate small molecule-RNA-protein interactome.
  • Maintain detailed records of experimental protocols and results in laboratory notebooks.
  • Clear presentation and efficient communication with cross-functional teams.
  • Manage internal and external resources to prioritize activities while balancing multiple priorities.

Education/Skills/Experience Requirements: 

  • PhD in Biochemistry, Molecular Biology or a related field and 0-4 years of postdoctoral research experience with a proven track record of scientific publications related to RNA biology.
  • Knowledge of molecular mechanisms of RNA processing.
  • Hands-on experience with in vitro RNA biochemical assays is highly desirable, including in vitro transcription, splicing, translation, decay assays.
  • Experience with core molecular biology techniques including constructs design, RNA purification, RT-PCR, qPCR, western blot, RNA/protein immunoprecipitation.
  • Experience with mammalian cell culture and stable cell line generation.
  • Experience with state-of-art quantitative proteomics techniques including SILAC, TMT, AP-MS.
  • Excellent organizational and communication skills with proven experience to work independently as well as in a multidisciplinary team.
  • Flexible and efficient with a problem-solving attitude in a fast-paced small company environment.

To Apply: Please send resume and cover letter to [email protected]

A COVER LETTER IS REQUIRED. Please explain why this job is of interest to you. Detail your strengths and weaknesses as they pertain to the requirements and responsibilities of this position.