SHENG2 - Postdoc

Posted on April 19,

Laboratory of RNA Biology headed by Prof. Joanna Kufel is looking for Postdoc (two positions) at the Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw for the project “Mechanistic study of transcription regulation in Arabidopsis”, funded by the National Science Centre Sheng2 grant.
Starting date: 01.06.2022, duration 3 years. 

Project description:
This project involves comprehensive studies of transcription regulation in Arabidopsis thaliana by three proteins, XRN3, DXO1 and RSA1. XRN3, a nuclear 5'-3' exoribonuclease, is involved in the torpedo transcription termination mechanism of RNA polymerase II. DXO1 is an enzyme, present in the nucleus and the cytoplasm, capable of removing the non-canonical NAD+ cap on RNAs, but its major functions are unrelated to catalytic activity. In turn, RSA1 is a nuclear protein that forms liquid-like condensates that contain components of the transcription machinery and RNA-related proteins. Our data suggest that these proteins interact physically and/or functionally and are associated with Pol II activity. The main objective is to dissect the exact mechanism of their joint contribution to transcription initiation, elongation and termination, possibly through condensate formation (liquid-liquid phase separation, LLPS) with the transcriptional machinery. We also want to assess the impact of transcriptional activity of these factors on cellular processes and plant physiology, including response to environmental stresses.

We will employ a broad range of high-throughput techniques (ChIP-seq, NET-seq, ChAR-seq), as well as in vitro and in vivo molecular biology, biochemical and genetic approaches to detect and visualize LLPS events, and to characterize the protein and RNA content of these structures. This research will provide in depth description of transcription regulation by these three factors and will establish the role of the LLPS phenomenon in this process.

Apply to Joanna Kufel via e-mail: [email protected] or [email protected] (only candidates considered suitable for the post will be informed by return e-mail)
Application deadline: 20.04.2022

Postdoc position: Employment contract full-time
Salary 8000 PLN/month gross salary, (expected net salary 6 000 PLN/month)

Recent Ph.D. degree in life sciences: molecular biology, genetics or related field (7 years or less after Ph.D.)
Knowledge of molecular biology of eukaryotic organisms.
Experience in RNA molecular biology and physiology, high-throughput NGS approaches and microscopy-based RNA and protein imaging.
Competence in bioinformatics analyses of transcriptomics data.
Publications in international peer-reviewed journals.
Very good oral and written communication skills in English.

Required documents:
CV with detailed description of research experience, list of publications and information about previous employment (if applicable)
Motivation letter
Copy of PhD (postdoc) diploma
Statement: “I give permission to the University of Warsaw, registered at the address of ul. Krakowskie Przedmieście 26/28, 00-927 Warszawa, to process my personal data for the purposes of carrying out the recruitment procedure, choosing the employee, and entering into an employment contract with the University of Warsaw, if applicable. I have been informed about my legal rights and obligations in relation to these actions. I acknowledge that providing the aforementioned personal data is done by me on a voluntary basis.“ (can be added to the CV)
Contact details and e-mail addresses of two people willing to provide a reference letter on request